Development and characterization of cheese spread fortified with flaxseed oil
DOI:
https://doi.org/10.5219/scifood.95Keywords:
flaxseed oil, cheese spread, omega-3 fortification, physicochemical properties, sensory evaluationAbstract
This study aimed to develop a healthier processed cheese spread by replacing milk fat with flaxseed oil, a rich source of omega-3 fatty acids. The research tested flaxseed oil substitution levels of 25%, 50%, 75%, and 100% to find the optimal balance between health benefits and product quality. Key analyses included titratable acidity, moisture content, pH, fat content, protein, ash content, microbiological growth, and sensory evaluation. The major finding includes that increasing flaxseed oil led to decreased initial acidity, but acidity increased during storage. Moisture content rose over 21 days initial moisture ranged 35.05–36.55%, slightly increasing during 21 days, with T4 and T3 maintaining the highest levels (36.77%) at later stages, and pH increased with more flaxseed oil, as Fresh samples displayed pH values ranging from 6.16 (T0) to 6.31 (T4), though 25% substitution showed similar pH to the control. Fat contents and ash contents significantly increased with flaxseed oil fortification. Microbial counts also rose, likely due to typical cheese breakdown organisms, as Fresh samples had initial TBC values ranging from 83 CFU/g (T0) to 91 CFU/g (T4). By Day 21, the TBC for T0 rose to 101 CFU/g, while T4 showed a more pronounced increase, reaching 116 CFU/g. Sensory tests showed colour changes to yellow and brown hues, but overall good acceptance (T3, T4), yielding lower initial scores due to the oil's distinct colour and flavor profile. T0 (control) and T1 (25% oil) maintained superior acceptability throughout. The study concludes that flaxseed oil can produce a healthier cheese spread with lower levels of saturated and trans fats while maintaining acceptable sensory qualities.
References
1. Fekete, M., Lehoczki, A., Kryczyk-Poprawa, A., Zábó, V., Varga, J., Bálint, M., Fazekas-Pongor, V., Csípő, T., Rząsa-Duran, E., & Varga, P. (2025). Functional Foods in Modern Nutrition Science: Mechanisms, Evidence, and Public Health Implications. Nutrients, 17(13), 2153. https://doi.org/10.3390/nu17132153 DOI: https://doi.org/10.3390/nu17132153
2. Mohtarami, F., Rashidi, Z., & Pirsa, S. (2022). Extraction of flaxseed and Plantago Psyllium mucilage: Investigation of rheological properties and efficiency as a fat substitute for the production of low‐calorie cookies. Journal of Food Processing and Preservation, 46(11). https://doi.org/10.1111/jfpp.16964 DOI: https://doi.org/10.1111/jfpp.16964
3. Azad, M., Nadeem, M., Gulzar, N., & Imran, M. (2021). Impact of fractionation on fatty acids composition, phenolic compounds, antioxidant characteristics of olein and super olein fractions of flaxseed oil. Journal of Food Processing and Preservation, 45(4). https://doi.org/10.1111/jfpp.15369 DOI: https://doi.org/10.1111/jfpp.15369
4. Daoust, L. (2024). Food reformulation and public health. Nature Food, 5(4), 274–274. https://doi.org/10.1038/s43016-024-00972-5 DOI: https://doi.org/10.1038/s43016-024-00972-5
5. Upadhyay, N., Kamble, K., Tiwari, S., Meena, S. K., Malhotra, R., Singh, A. K., & Kaur, C. (2024). Novel avenue of carotenoids & omega-3 rich carrot waste extract in innovative table spread: optimization (based on texture and sensory) and characterization. Discover Food, 4(1). https://doi.org/10.1007/s44187-024-00141-4 DOI: https://doi.org/10.1007/s44187-024-00141-4
6. Ghosal, S., Bhattacharyya, D. K., & Bhowal, J. (2022). Production, characterization, and storage stability of nutritionally enriched flaxseed‐based spread. Journal of Food Processing and Preservation, 46(5). https://doi.org/10.1111/jfpp.16574 DOI: https://doi.org/10.1111/jfpp.16574
7. Florczuk, A., Dąbrowska, A., & Aljewicz, M. (2022). An evaluation of the effect of curdlan and scleroglucan on the functional properties of low-fat processed cheese spreads. LWT, 163, 113564. https://doi.org/10.1016/j.lwt.2022.113564 DOI: https://doi.org/10.1016/j.lwt.2022.113564
8. Sala-Vila, A., Fleming, J., Kris-Etherton, P., & Ros, E. (2022). Impact of α-Linolenic Acid, the Vegetable ω-3 Fatty Acid, on Cardiovascular Disease and Cognition. Advances in Nutrition, 13(5), 1584–1602. https://doi.org/10.1093/advances/nmac016 DOI: https://doi.org/10.1093/advances/nmac016
9. Baker, E. J., Cumberford, G., & Hanaway, P. (2025). Recognizing the Health Benefits of Plant‐Sourced Omega‐3 Stearidonic Acid: Exploring Its Complementary Role to Preformed EPA/DHA. Lipids, 60(6), 435–442. https://doi.org/10.1002/lipd.12452 DOI: https://doi.org/10.1002/lipd.12452
10. Dong, S., Wang, Y., Bian, J., Chen, H., Dong, J., Zhu, J., Zhang, T., Du, Q., & Zhao, R. (2024). The effect of omega-3 Polyunsaturated Fatty Acid (PUFA) prescription preparations on the prevention of clinical cardiovascular disease: a meta-analysis of RCTs. Nutrition Journal, 23(1). https://doi.org/10.1186/s12937-024-01051-y DOI: https://doi.org/10.1186/s12937-024-01051-y
11. Almasi, K., Esnaashari, S. S., Khosravani, M., & Adabi, M. (2021). Yogurt fortified with omega‐3 using nanoemulsion containing flaxseed oil: Investigation of physicochemical properties. Food Science & Nutrition, 9(11), 6186–6193. https://doi.org/10.1002/fsn3.2571 DOI: https://doi.org/10.1002/fsn3.2571
12. Koçak, M. Z. (2024). Phenolic Compounds, Fatty Acid Composition, and Antioxidant Activities of Some Flaxseed (Linum usitatissimum L.) Varieties: A Comprehensive Analysis. Processes, 12(4), 689. https://doi.org/10.3390/pr12040689 DOI: https://doi.org/10.3390/pr12040689
13. Azarpazhooh, E., Rashidi, H., Sharayei, P., Behmadi, H., & Ramaswamy, H. S. (2021). Effect of flaxseed-mucilage and Stevia on physico-chemical, antioxidant and sensorial properties of formulated cocoa milk. Food Hydrocolloids for Health, 1, 100017. https://doi.org/10.1016/j.fhfh.2021.100017 DOI: https://doi.org/10.1016/j.fhfh.2021.100017
14. Xia, H., Wang, Y., Shi, X., Liao, W., Wang, S., Sui, J., & Sun, G. (2023). Beneficial Effects of Dietary Flaxseed Oil through Inflammation Pathways and Gut Microbiota in Streptozotocin-Induced Diabetic Mice. Foods, 12(17), 3229. https://doi.org/10.3390/foods12173229 DOI: https://doi.org/10.3390/foods12173229
15. El-Waseif, M., Saed, B., Fahmy, H., Sabry, A., Shaaban, H., Abdelgawad, M., Amin, A., & Farouk, A. (2022). Mayonnaise Enriched with Flaxseed Oil: Omega-3 Fatty Acids Content, Sensory Quality and Stability during the Storage. Foods, 11(15), 2288. https://doi.org/10.3390/foods11152288 DOI: https://doi.org/10.3390/foods11152288
16. Surkova, S. A., Slozhenkina, M. I., Gorlov, I. F., Mosolova, N. I., Tkachenkova, N. A., & Grebennikova, J. D. (2022). Vegetable oils in dairy production: perspectives, food safety and guarantee of sustainable development of the food system. IOP Conference Series: Earth and Environmental Science, 981(2), 022090. https://doi.org/10.1088/1755-1315/981/2/022090 DOI: https://doi.org/10.1088/1755-1315/981/2/022090
17. Swapiti, S. A., Hosseini, S., & Khandouzi, N. (2020). Effect of flaxseed oil supplementation on the erythrocyte membrane fatty acid composition and inflammatory markers in patients with coronary artery disease. Lipids in Health and Disease, 19(1), 92. https://doi.org/10.1186/s12944-020-01279-8
18. López-Toledo, S., Pineda De la Cruz, M., Gutiérrez-Hurtado, I., Gijón-Soriano, A., Martínez-Martínez, E., Valencia-Santiago, C., Orellana-Centeno, J., Ramírez-García, S., & Pacheco-Cruz, R. (2025). Flaxseed Improves Glucose and Lipid Metabolism in Mexican Subjects with Type 2 Diabetes: A Parallel Randomized Clinical Trial. Nutrients, 17(4), 709. https://doi.org/10.3390/nu17040709 DOI: https://doi.org/10.3390/nu17040709
19. Gawałek, J., Cais-Sokolińska, D., & Teichert, J. (2025). Mare’s and Cow’s Milk Fortified with Flaxseed Oil Through Freeze-Drying Microencapsulation: Physicochemical and Nutritional Properties. Foods, 14(2), 280. https://doi.org/10.3390/foods14020280 DOI: https://doi.org/10.3390/foods14020280
20. Aydeniz Guneser, B. (2022). Characterization of physicochemical, rheological, aroma, and sensory properties of spreadable processed cheese supplemented with chia, quinoa, and teff seeds. Mljekarstvo, 73(1), 27–37. https://doi.org/10.15567/mljekarstvo.2023.0104 DOI: https://doi.org/10.15567/mljekarstvo.2023.0104
21. Renuka, S., & Ramasamy, D. (2016). Fortification of omega-3 fatty acids in processed cheese spread. International Journal of Science, Environment and Technology, 5(4), 2557–2565. https://www.ijset.net/journal/1195.pdf
22. Ashraf, U., Sameen, A., Ashraf, J. Z., Tuseef, M., Khalid, R., Abbas, S. M. N., Sultan, M. M., Rafique, M., & Gull, T. (2023). Development and evaluation of processed cheese spread supplemented with ripened Cheddar cheese. Journal of Food and Biological Sciences, 2(1). URL: https://jfbs.juw.edu.pk/index.php/jfbs/article/view/14 DOI: https://doi.org/10.37962/jfbs.v2i1.14
23. Liu, T., Wu, J., Aziz, T., Xue, R., Khowdiary, M. M., & Yang, Z. (2024). Changes of physicochemical and functional properties of processed cheese made with natural cheddar and mozzarella cheeses during refrigerated storage. Scientific Reports, 14(1). https://doi.org/10.1038/s41598-024-53748-z DOI: https://doi.org/10.1038/s41598-024-53748-z
24. Alqahtani, N. K., Alnemr, T. M., Alqattan, A. M., Aleid, S. M., & Habib, H. M. (2023). Physicochemical and Sensory Properties and Shelf Life of Block-Type Processed Cheeses Fortified with Date Seeds (Phoenix dactylifera L.) as a Functional Food. Foods, 12(3), 679. https://doi.org/10.3390/foods12030679 DOI: https://doi.org/10.3390/foods12030679
25. Ahmed, S., Awad, R., Ali, M., & Rashid, M. (2019). Chemical and biological studies on spreadable processed cheese made using flaxseed oil as butter substitute. Al-Azhar Journal of Agricultural Research, 44(2), 35–48. https://doi.org/10.21608/ajar.2019.101823 DOI: https://doi.org/10.21608/ajar.2019.101823
26. Gurdian, C., Reyes, V., Kyereh, E., Bonilla, F., Galindo, C., Chouljenko, A., Solval, K. M., Boeneke, C., King, J. M., & Sathivel, S. (2017). Incorporating flaxseed (linum usitatissimum) oil into queso blanco at different stages of the cheese manufacturing process. Journal of Food Processing and Preservation, 41(6), e13279. https://doi.org/10.1111/jfpp.13279 DOI: https://doi.org/10.1111/jfpp.13279
27. Lehaçani, S. M., & Al-Abdullah, B. (2023). Characterisation of soft white cheese fortified with flaxseed oil to enhance its quality, lipid profile and health benefits. Rivista Italiana delle Sostanze Grasse, 100(3). https://www.innovhub-ssi.it/kdocs/2108138/2023_risg_3.pdf
28. Nasirpour-Tabrizi, P., Azadmard-Damirchi, S., Hesari, J., Khakbaz Heshmati, M., & Savage, G. P. (2020). Rheological and physicochemical properties of novel low-fat emulgels containing flaxseed oil as a rich source of ω-3 fatty acids. LWT, 133, 110107. https://doi.org/10.1016/j.lwt.2020.110107 DOI: https://doi.org/10.1016/j.lwt.2020.110107
29. Kumar, M., & Sharma, B. D. (2004). The storage stability and textural, physico-chemical and sensory quality of low-fat ground pork patties with Carrageenan as fat replacer. International Journal of Food Science and Technology, 39(1), 31–42. https://doi.org/10.1111/j.1365-2621.2004.00743.x DOI: https://doi.org/10.1111/j.1365-2621.2004.00743.x
30. Chatziantoniou, S. E., Thomareis, A. S., & Kontominas, M. G. (2015). Effect of chemical composition on physico-chemical, rheological and sensory properties of spreadable processed whey cheese. European Food Research and Technology, 241(6), 737–748. https://doi.org/10.1007/s00217-015-2499-6 DOI: https://doi.org/10.1007/s00217-015-2499-6
31. Alsaleem, K., Hamouda, M., Alayouni, R., Elfaruk, M., & Hammam, A. (2023). Effect of Skim Milk Powder and Whey Protein Concentrate Addition on the Manufacture of Probiotic Mozzarella Cheese. Fermentation, 9(11), 948. https://doi.org/10.3390/fermentation9110948 DOI: https://doi.org/10.3390/fermentation9110948
32. Abbas, K. A., Abdelmontaleb, H. S., Hamdy, S. M., & Aït-Kaddour, A. (2021). Physicochemical, Functional, Fatty Acids Profile, Health Lipid Indices, Microstructure and Sensory Characteristics of Walnut-Processed Cheeses. Foods, 10(10), 2274. https://doi.org/10.3390/foods10102274 DOI: https://doi.org/10.3390/foods10102274
33. Ehsannia, S., & Sanjabi, M. R. (2016). Physicochemical, microbiological and spoilage analysis of probiotic processed cheese analogues with reduced emulsifying salts during refrigerated storage. Journal of Food Science and Technology, 53(2), 996–1003. https://doi.org/10.1007/s13197-015-2159-7 DOI: https://doi.org/10.1007/s13197-015-2159-7
34. Kukhtyn, M., Arutiunian, D., Pokotylo, O., Kravcheniuk, K., Salata, V., Horiuk, Y., Karpyk, H., & Dalievska, D. (2024). Microbiological characteristics of hard cheese with flax seeds. Potravinarstvo Slovak Journal of Food Sciences, 18, 281–296. https://doi.org/10.5219/1956
35. Irfan, S., Murtaza, M. A., Mueen ud Din, G., Hafiz, I., Murtaza, M. S., Rafique, S., Ameer, K., Abrar, M., & Mohamed Ahmed, I. A. (2022). Physicochemical, microbial, and functional attributes of processed Cheddar cheese fortified with olive oil–whey protein isolate emulsion. Food Science & Nutrition, 11(3), 1247–1256. https://doi.org/10.1002/fsn3.3159 DOI: https://doi.org/10.1002/fsn3.3159
36. Todaro, M., Palmeri, M., Settanni, L., Scatassa, M. L., Mazza, F., Bonanno, A., & Di Grigoli, A. (2017). Effect of refrigerated storage on microbiological, chemical and sensory characteristics of a ewes’ raw milk stretched cheese. Food Packaging and Shelf Life, 11, 67–73. https://doi.org/10.1016/j.fpsl.2017.01.005 DOI: https://doi.org/10.1016/j.fpsl.2017.01.005
37. Rynne, N. M., Beresford, T. P., Guinee, T. P., Sheehan, E., Delahunty, C. M., & Kelly, A. L. (2008). Effect of high-pressure treatment of 1 day-old full-fat Cheddar cheese on subsequent quality and ripening. Innovative Food Science & Emerging Technologies, 9(4), 429–440. https://doi.org/10.1016/j.ifset.2008.02.004 DOI: https://doi.org/10.1016/j.ifset.2008.02.004
38. Yenipazar, H., & Şahin‐Yeşilçubuk, N. (2022). Effect of packaging and encapsulation on the oxidative and sensory stability of omega‐3 supplements. Food Science & Nutrition, 11(3), 1426–1440. https://doi.org/10.1002/fsn3.3182 DOI: https://doi.org/10.1002/fsn3.3182
39. Ribeiro, A. P. B., Basso, R. C., Grimaldi, R., Gioielli, L. A., dos Santos, A. O., Cardoso, L. P., & Guaraldo Gonçalves, L. A. (2009). Influence of chemical interesterification on thermal behavior, microstructure, polymorphism and crystallization properties of canola oil and fully hydrogenated cottonseed oil blends. Food Research International, 42(8), 1153–1162. https://doi.org/10.1016/j.foodres.2009.05.016 DOI: https://doi.org/10.1016/j.foodres.2009.05.016
40. Kukhtyn, M., Arutiunian, D., Pokotylo, O., Kravcheniuk, K., Salata, V., Horiuk, Y., Karpyk, H., & Dalievska, D. (2024). Microbiological characteristics of hard cheese with flax seeds. Potravinarstvo Slovak Journal of Food Sciences, 18, 281–296. https://doi.org/10.5219/1956 DOI: https://doi.org/10.5219/1956
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Zaigham Abbas, Abdur Rafay Saeed (Author)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles are published under the Creative Commons Attribution–NonCommercial–NoDerivatives 4.0 International License (CC BY-NC-ND 4.0).
This license permits non-commercial use, sharing, distribution, and reproduction of the work in any medium or format, provided that:
-
appropriate credit is given to the author(s) and the original publication in Scifood,
-
a link to the license is provided,
-
the work is not modified, adapted, or transformed.






