Tofu processing: selected parameters from the seeds to the final products and by-products

Authors

  • Daniela Lukáčová The Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Science, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421 37 641 5825 , Slovak University of Agriculture in Nitra image/svg+xml Author
  • Martina Fikselová The Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Science, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421 37 641 5825 , Slovak University of Agriculture in Nitra image/svg+xml Author
  • Radoslav Židek The Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Science, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421 37 641 5825 , Slovak University of Agriculture in Nitra image/svg+xml Author
  • Jozef Čapla The Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Science, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421 37 641 4371 , Slovak University of Agriculture in Nitra image/svg+xml Author
  • Peter Zajác The Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Science, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421 37 641 4371 , Slovak University of Agriculture in Nitra image/svg+xml Author

DOI:

https://doi.org/10.5219/scifood.101

Keywords:

soybean, tofu, yield, amino acids, hydration

Abstract

The efficiency of soy food production, particularly tofu, depends on a complex of physicochemical properties of soybean seeds (Glycine max). This study analyzes 187 soybean samples to evaluate the primary determinants of tofu yield/production. The main objective was to analyze the relationships among selected physicochemical properties of soybeans and tofu production. The methods applied were Near-Infrared Spectroscopy (NIR), physical parameters (hydration coefficient, thousand seed weight [TSW]), and correlation and regression analyses to identify relationships among hydration potential, seed properties, dry matter content, and the amino acid profile. Our results show that the hydration potential is strongly positively associated with seed dry matter content, indicating that dry matter is a critical predictor of water absorption efficiency. Raw tofu yield demonstrated a clear positive correlation with the physical properties of the seeds, including TSW and the hydration coefficient. An original contribution of this work is the identification of a negative amino-acid signature associated with reduced tofu yield. Aspartic acid, histidine, alanine, and glycine showed significant negative correlations with tofu raw yield, whereas proline content showed a slight positive association.

References

1. OECD-FAO Agricultural Outlook 2025-2034. In OECD-FAO Agricultural Outlook. OECD Publishing. https://doi.org/10.1787/601276cd-en

2. Wang, D., Chen, G., Yang, B., Chen, X., Song, J., Kong, X., & Kan, J. (2021). Kinetic study on soybean hydration during soaking and resulting softening kinetic during cooking. Journal of Food Science, 87(1), 266–279. https://doi.org/10.1111/1750-3841.15984

3. Li, X., Liu, X., Hua, Y., Chen, Y., Kong, X., & Zhang, C. (2019). Effects of water absorption of soybean seed on the quality of soymilk and the release of flavor compounds. RSC Advances, 9(6), 2906–2918. https://doi.org/10.1039/c8ra08029a

4. Hsu, K. H., Kim, C. J., & Wilson, L. A. (1983). Factors affecting water uptake of soybeans during soaking. Cereal Chemistry, 60(3), 208–211.

5. Gowen, A., Abu-Ghannam, N., Frias, J., & Oliveira, J. (2007). Influence of pre-blanching on the water absorption kinetics of soybeans. Journal of Food Engineering, 78(3), 965–971. https://doi.org/10.1016/j.jfoodeng.2005.12.009

6. Perera, D., Devkota, L., Shrestha, S., Bhattarai, S. P., Panozzo, J., & Dhital, S. (2026). Hard-to-cook (HTC) phenomenon associated changes in protein and lipid profile of faba and adzuki beans. Food Hydrocolloids, 171, 111803. https://doi.org/10.1016/j.foodhyd.2025.111803

7. Rekha, C. R., & Vijayalakshmi, G. (2011). Influence of processing parameters on the quality of soycurd (tofu). Journal of Food Science and Technology, 50(1), 176–180. https://doi.org/10.1007/s13197-011-0245-z

8. Bhardwaj, H. L., Bhagsari, A. S., Joshi, J. M., Rangappa, M., Sapra, V. T., & Rao, M. S. S. (1999). Yield and Quality of Soymilk and Tofu Made from Soybean Genotypes Grown at Four Locations. Crop Science, 39(2), 401–405. https://doi.org/10.2135/cropsci1999.0011183x0039000200017x

9. Saio, K., Kamiya, M., & Watanabe, T. (1969). Food Processing Characteristics of Soybean 11S and 7S Proteins. Agricultural and Biological Chemistry, 33(9), 1301–1308. https://doi.org/10.1080/00021369.1969.10859465

10. Wang, H. L., & Cavins, J. F. (1989). Yield and amino acid composition of fractions obtained during tofu production. Cereal Chemistry, 66(5), 359–361.

11. Zhu, Z., Chen, S., Wu, X., Xing, C., & Yuan, J. (2018). Determination of soybean routine quality parameters using near‐infrared spectroscopy. Food Science & Nutrition, 6(4), 1109–1118. https://doi.org/10.1002/fsn3.652

12. Meyer, C. J., Steudle, E., & Peterson, C. A. (2006). Patterns and kinetics of water uptake by soybean seeds. Journal of Experimental Botany, 58(3), 717–732. https://doi.org/10.1093/jxb/erl244

13. Smith, A. K., Nash, A. M., & Wilson, L. I. (1961). Water absorption of soybeans. Journal of the American Oil Chemists’ Society, 38(3), 120–123. https://doi.org/10.1007/bf02641230

14. Gourkhede, P. H., Baraptre, D. D., & Pendke, M. S. (2024). Studies on effect of seed hydration value of various soybean varieties with respect to soil types and moisture regimes. International Journal of Advanced Biochemistry Research, 8(4S), 479–491. https://doi.org/10.33545/26174693.2024.v8.i4sf.1035

15. Hendrawati, T. Y., Audini, K., Ismiyati, Ramadhan, A. I., & Gustia, H. (2021). Effects and characterization of different soybean varieties in yield and organoleptic properties of tofu. Results in Engineering, 11, 100238. https://doi.org/10.1016/j.rineng.2021.100238

16. Guan, X., Zhong, X., Lu, Y., Du, X., Jia, R., Li, H., & Zhang, M. (2021). Changes of Soybean Protein during Tofu Processing. Foods, 10(7), 1594. https://doi.org/10.3390/foods10071594

17. Zuo, F., Chen, Z., Shi, X., Wang, R., & Guo, S. (2016). Yield and textural properties of tofu as affected by soymilk coagulation prepared by a high-temperature pressure cooking process. Food Chemistry, 213, 561–566. https://doi.org/10.1016/j.foodchem.2016.07.008

18. Döttinger, C. A., Steige, K. A., Hahn, V., Bachteler, K., Leiser, W. L., Zhu, X., & Würschum, T. (2025). Unravelling the genetic architecture of soybean tofu quality traits. Molecular Breeding, 45(1). https://doi.org/10.1007/s11032-024-01529-x

19. Benassi, V. de T., Yamashita, F., & Prudencio, S. H. (2011). A statistical approach to define some tofu processing conditions. Food Science and Technology (Campinas), 31(4), 897–904. https://doi.org/10.1590/s0101-20612011000400011

20. Alpaslan, M., & Hayta, M. (2002). Hydration properties, soymilk and okara yield of soybean affected by agronomic factors. Nahrung/Food, 46(3), 141. https://doi.org/10.1002/1521-3803(20020501)46:3<141::aid-food141>3.0.co;2-8

21. Joo, K. H., Kerr, W. L., & Cavender, G. A. (2023). The Effects of Okara Ratio and Particle Size on the Physical Properties and Consumer Acceptance of Tofu. Foods, 12(16), 3004. https://doi.org/10.3390/foods12163004

22. Bainy, E. M., Corredig, M., Poysa, V., Woodrow, L., & Tosh, S. (2010). Assessment of the effects of soy protein isolates with different protein compositions on gluten thermosetting gelation. Food Research International, 43(6), 1684–1691. https://doi.org/10.1016/j.foodres.2010.05.010

23. Vasconcellos, F. C. S., Woiciechowski, A. L., Soccol, V. T., Mantovani, D., & Soccol, C. R. (2014). Antimicrobial and antioxidant properties of β-conglycinin and glycinin from soy protein isolate. International Journal of Current Microbiology and Applied Sciences, 3(8), 144–157.

24. McClure, T., Cocuron, J.-C., Osmark, V., McHale, L. K., & Alonso, A. P. (2017). Impact of Environment on the Biomass Composition of Soybean (Glycine max) seeds. Journal of Agricultural and Food Chemistry, 65(32), 6753–6761. https://doi.org/10.1021/acs.jafc.7b01457

25. de Borja Reis, A. F., Tamagno, S., Moro Rosso, L. H., Ortez, O. A., Naeve, S., & Ciampitti, I. A. (2020). Historical trend on seed amino acid concentration does not follow protein changes in soybeans. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-74734-1

26. Mujoo, R., Trinh, D. T., & Ng, P. K. W. (2003). Characterization of storage proteins in different soybean varieties and their relationship to tofu yield and texture. Food Chemistry, 82(2), 265–273. https://doi.org/10.1016/s0308-8146(02)00547-2

27. Asrat, U., & Gudisa, A. (2025). Qualitative variabilities among different soybean ( Glycine max (L.) Merrill) varieties. Cogent Food & Agriculture, 11(1). https://doi.org/10.1080/23311932.2025.2562166

28. Wu, C., Hua, Y., Chen, Y., Kong, X., & Zhang, C. (2016). Effect of 7S/11S ratio on the network structure of heat-induced soy protein gels: a study of probe release. RSC Advances, 6(104), 101981–101987. https://doi.org/10.1039/c6ra22388e

29. Dang, Y., Ren, J., Guo, Y., Yang, Q., Liang, J., Li, R., Zhang, R., Yang, P., Gao, X., & Du, S. (2023). Structural, functional properties of protein and characteristics of tofu from small-seeded soybeans grown in the Loess Plateau of China. Food Chemistry: X, 18, 100689. https://doi.org/10.1016/j.fochx.2023.100689

30. Rohrer, K., Whitfield, F., Aussanasuwannakul, A., Ningrum, A., Hugi, C., & Breitenmoser, L. (2025). Comparative Screening Life Cycle Assessments of Okara Valorisation Scenarios. Environments, 12(3), 93. https://doi.org/10.3390/environments12030093

31. Santos, V. A. Q, Nascimento, C.G., Schmidt, C.A.P., Mantovani,D., Dekker,R.F.H., Cunha, M.A.A. (2018). Solid-state fermentation of soybean okara: Isoflavones biotransformation, antioxidant activity and enhancement of nutritional quality, LWT, 92, 509-515, https://doi.org/10.1016/j.lwt.2018.02.067

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Published

2026-02-25 — Updated on 2026-02-25

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Vol. 20 (2026): Scifood

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Copyright (c) 2026 Daniela Lukáčová, Martina Fikselová, Radoslav Židek, Jozef Čapla, Peter Zajác (Author)

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How to Cite

Tofu processing: selected parameters from the seeds to the final products and by-products. (2026). Scifood, 20(1), 135-145. https://doi.org/10.5219/scifood.101

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