The physicochemical and sensory profiles of spreadable dairy matrix obtained from kidney bean milk-like extract and cow milk blends
DOI:
https://doi.org/10.5219/scifood.39Keywords:
cow’s milk, white kidney bean, milk-like extract, dairy matrix, hybrid cheeseAbstract
As consumer interest in plant-based alternatives to traditional dairy products increases, incorporating legumes such as white beans into dairy products presents a promising opportunity for developing nutritious and palatable options. This study investigates the physicochemical and sensory properties of a dairy matrix (DM) created by mixing cow's milk with a milk-like extract of white kidney beans in various ratios. Dried white kidney beans (Phaseolus vulgaris) were sourced from a local market in Talas, Kyrgyzstan. The beans were soaked overnight, washed, ground with added water, and filtered to obtain a milk-like extract (BMLE), stored at 4°C. The DM was prepared by mixing BMLE with cow’s milk in five proportions (0%:100%, 30%:70%, 50%:50%, 70%:30%, and 100%:0%) and pasteurizing at 95°C for 25 minutes. Adding 40% CaCl2 at 95°C resulted in protein precipitation, forming a curd-like matrix, which was then self-pressed to a moisture content of 68–79% and stored at 4°C. A spreadable dairy matrix was formed in samples containing up to 50% BMLE. These experimental DMs exhibited significantly higher spreadability than the control (100% cow’s milk). Higher BMLE content correlated with increased water holding capacity (WHC) and spreadability. Samples containing BMLE had higher acidity levels compared to the control. Color analysis revealed that samples with BMLE had a more pronounced reddish hue (a*), whereas the control exhibited stronger yellowish and bluish tones (b*). Sensory analysis indicated distinct taste differences between experimental and control samples, although overall acceptability remained comparable.
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References
1. Lumsden, C. L., Jägermeyr, J., Ziska, L., & Fanzo, J. (2024). Critical overview of the implications of a global protein transition in the face of climate change: Key unknowns and research imperatives. In One Earth (Vol. 7, Issue 7, pp. 1187–1201). Elsevier BV. https://doi.org/10.1016/j.oneear.2024.06.013
2. Srenuja, D., Hema, V., Anand, M. T., Mohan, R. J., & Vidyalakshmi, R. (2023). Kidney bean: Protein’s treasure trove and creates avenues for a healthy lifestyle. In Legume Science (Vol. 5, Issue 3). Wiley. https://doi.org/10.1002/leg3.193
3. Plamada, D., Teleky, B.-E., Nemes, S. A., Mitrea, L., Szabo, K., Călinoiu, L.-F., Pascuta, M. S., Varvara, R.-A., Ciont, C., Martău, G. A., Simon, E., Barta, G., Dulf, F. V., Vodnar, D. C., & Nitescu, M. (2023). Plant-Based Dairy Alternatives—A Future Direction to the Milky Way. In Foods (Vol. 12, Issue 9, p. 1883). MDPI AG. https://doi.org/10.3390/foods12091883
4. Jaeger, S. R., Jin, D., & Roigard, C. M. (2024). Plant-Based Alternatives Need Not Be Inferior: Findings from a Sensory and Consumer Research Case Study with Cream Cheese. In Foods (Vol. 13, Issue 4, p. 567). MDPI AG. https://doi.org/10.3390/foods13040567
5. Abd El-Khair, A. E.-A., Ahmed, A., & Ateteallah, A. (2023). Production and Evaluation of Soft Sheep’s Milk Cheese Analogues Supplemented With Quinoa Seed Flour and Xanthan Gum. In Journal of Sohag Agriscience (JSAS) (Vol. 8, Issue 2, pp. 269–281). Egypts Presidential Specialized Council for Education and Scientific Research. https://doi.org/10.21608/jsasj.2023.334541
6. Narote, A. D., Casson, A., Giovenzana, V., Pampuri, A., Tugnolo, A., Beghi, R., & Guidetti, R. (2025). Integrating environmental, nutritional, and economic dimensions in Food choices: A case study on legume vs. meat-based burger patties. In Journal of Agriculture and Food Research (Vol. 21, p. 101820). Elsevier BV. https://doi.org/10.1016/j.jafr.2025.101820
7. Nolan, R., Shannon, O. M., Robinson, N., Joel, A., Houghton, D., & Malcomson, F. C. (2020). It’s No Has Bean: A Review of the Effects of White Kidney Bean Extract on Body Composition and Metabolic Health. In Nutrients (Vol. 12, Issue 5, p. 1398). MDPI AG. https://doi.org/10.3390/nu12051398
8. Mohd Shukri, A., Alias, A. K., Murad, M., Yen, K., & Cheng, L. (2021). A review of natural cheese and imitation cheese. In Journal of Food Processing and Preservation (Vol. 46, Issue 1). Hindawi Limited. https://doi.org/10.1111/jfpp.16112
9. Bachmann, H.-P. (2001). Cheese analogues: a review. In International Dairy Journal (Vol. 11, Issues 4–7, pp. 505–515). Elsevier BV. https://doi.org/10.1016/s0958-6946(01)00073-5
10. Genet, B. M. L., Sedó Molina, G. E., Wätjen, A. P., Barone, G., Albersten, K., Ahrné, L. M., Hansen, E. B., & Bang-Berthelsen, C. H. (2023). Hybrid Cheeses—Supplementation of Cheese with Plant-Based Ingredients for a Tasty, Nutritious and Sustainable Food Transition. In Fermentation (Vol. 9, Issue 7, p. 667). MDPI AG. https://doi.org/10.3390/fermentation9070667
11. Tilekeyev, K., Mogilevskii, R., Abdrazakova, N., & Dzhumaeva, S. (2018). Production and Exports of Kidney Beans in the Kyrgyz Republic: Value Chain Analysis. In SSRN Electronic Journal. Elsevier BV. https://doi.org/10.2139/ssrn.3807836
12. Mulet-Cabero, A.-I., Torres-Gonzalez, M., Geurts, J., Rosales, A., Farhang, B., Marmonier, C., Ulleberg, E. K., Hocking, E., Neiderer, I., Gandolfi, I., Anderson, L., Brader, L., Vermaak, M., Cameron, M., Myrup Christensen, M., Haryono, R., & Peters, S. (2024). The Dairy Matrix: Its Importance, Definition, and Current Application in the Context of Nutrition and Health. In Nutrients (Vol. 16, Issue 17, p. 2908). MDPI AG. https://doi.org/10.3390/nu16172908
13. Tosif, M. M., Najda, A., Bains, A., Krishna, T. C., Chawla, P., Dyduch-Siemińska, M., Klepacka, J., & Kaushik, R. (2021). A Comprehensive Review on the Interaction of Milk Protein Concentrates with Plant-Based Polyphenolics. In International Journal of Molecular Sciences (Vol. 22, Issue 24, p. 13548). MDPI AG. https://doi.org/10.3390/ijms222413548
14. Tumbarski, Y., Yanakieva, V., Ivanov, I., Ivanova, M., Ivanova, P., & Parzhanova, A. (2024). Design of prebiotic cheese spreads enriched with biologically active compounds. In P. Mollov, G. Ivanov, G. Kostov, K. Mihalev, & S. Dragoev (Eds.), BIO Web of Conferences (Vol. 102, p. 01007). EDP Sciences. https://doi.org/10.1051/bioconf/202410201007
15. Farheen, S. A., & R, N. (2024). Development and Evaluation of Value-Added Cheese Spread using Red Cabbage Microgreens and Flaxseeds. In International Journal for Research in Applied Science and Engineering Technology (Vol. 12, Issue 4, pp. 3149–3154). International Journal for Research in Applied Science and Engineering Technology (IJRASET). https://doi.org/10.22214/ijraset.2024.60687
16. Delmonte, A., Cortez-Latorre, J. D., & Rozycki, S. D. (2022). Whey Revalorization through the Development of Spreadable Cheeses. In ESPOCH Congresses: The Ecuadorian Journal of S.T.E.A.M. (Vol. 2, Issue 1, pp. 228–244). Knowledge E DMCC. https://doi.org/10.18502/espoch.v2i2.11197
17. Tharayil Udayarajan, C., Mohan, K., Pulliyil Muhammed, R., Mendez Ts, T., & Nisha, P. (2024). Development and Optimization of Vegan Cheese Spread: Studies in Rheology and Microstructure. In Journal of Culinary Science & Technology (pp. 1–19). Informa UK Limited. https://doi.org/10.1080/15428052.2024.2359448
18. Meghrabi, S., & Yamani, M. I. (2023). Physicochemical and Sensory Characteristics of a New Milk Substitute from Dry White Kidney Bean. In Food Science and Technology (Vol. 11, Issue 4, pp. 218–225). Horizon Research Publishing Co., Ltd. https://doi.org/10.13189/fst.2023.110404
19. International Organization for Standardization. (2004). Cheese and processed cheese — Determination of the total solids content (Reference method) (ISO Standard No. 5534:2004). https://www.iso.org/standard/35249.html
20. International Organization for Standardization. (2022). Cheese and processed cheese products — Determination of fat content — Gravimetric method (Reference method) (ISO Standard No. ISO 23319:2022). https://www.iso.org/standard/75227.html
21. International Organization for Standardization. (2024). Milk and milk products — Determination of lactose content by high-performance liquid chromatography (reference method) (ISO Standard No. 22662:2024). https://www.iso.org/standard/84827.html
22. International Organization for Standardization. (2004). Milk — Determination of nitrogen content — Part 3: Block-digestion method (Semi-micro rapid routine method) (ISO Standard No. 8968-3:2004). https://www.iso.org/standard/35214.html
23. International Organization for Standardization. (2005). Animal feeding stuffs — Determination of amino acids content (ISO Standard No. 13903:2005). https://www.iso.org/standard/37258.html
24. Mileriene, J., Serniene, L., Henriques, M., Gomes, D., Pereira, C., Kondrotiene, K., Kasetiene, N., Lauciene, L., Sekmokiene, D., & Malakauskas, M. (2021). Effect of liquid whey protein concentrate–based edible coating enriched with cinnamon carbon dioxide extract on the quality and shelf life of Eastern European curd cheese. In Journal of Dairy Science (Vol. 104, Issue 2, pp. 1504–1517). American Dairy Science Association. https://doi.org/10.3168/jds.2020-18732
25. International Organization for Standardization. (2014). Sensory analysis — Methodology — General guidance for conducting hedonic tests with consumers in a controlled area (ISO Standard No. 11136:2014). https://www.iso.org/standard/50125.html
26. Ibaratkan Kurbanova, 1, Lina Lauciene, et al. (2024). Physicochemical, Sensory, and Microbiological Analysis of Fermented Drinks Made from White Kidney Bean Extract and Cow's Milk Blends during Refrigerated Storage. Microorganisms. (Vol. 12, Issue 9, p.1832). https://doi.org/10.3390/microorganisms12091832
27. Aydar, E. F., Mertdinç, Z., Demircan, E., Koca Çetinkaya, S., & Özçelik, B. (2023). Kidney bean (Phaseolus vulgaris L.) milk substitute as a novel plant-based drink: Fatty acid profile, antioxidant activity, in-vitro phenolic bio-accessibility and sensory characteristics. In Innovative Food Science & Emerging Technologies (Vol. 83, p. 103254). Elsevier BV. https://doi.org/10.1016/j.ifset.2022.103254
28. Geng, Y., Du, X., Jia, R., Zhu, Y., Lu, Y., Guan, X., Hu, Y., Zhu, X., & Zhang, M. (2024). Research Progress on Tofu Coagulants and Their Coagulation Mechanisms. In Foods (Vol. 13, Issue 21, p. 3475). MDPI AG. https://doi.org/10.3390/foods13213475
29. Lima Nascimento, L. G., Odelli, D., Fernandes de Carvalho, A., Martins, E., Delaplace, G., Peres de sá Peixoto Júnior, P., Nogueira Silva, N. F., & Casanova, F. (2023). Combination of Milk and Plant Proteins to Develop Novel Food Systems: What Are the Limits? In Foods (Vol. 12, Issue 12, p. 2385). MDPI AG. https://doi.org/10.3390/foods12122385
30. van Eck, E. B., Hofman, Z., van Eijnatten, E. J. M., Knol, J., Renes, I. B., & Abrahamse, E. (2024). Plant protein dominant enteral nutrition, containing soy and pea, is non-coagulating after gastric digestion in contrast to casein dominant enteral nutrition. In Food Research International (Vol. 197, p. 115162). Elsevier BV. https://doi.org/10.1016/j.foodres.2024.115162
31. Jäger, R., Abou Sawan, S., Purpura, M., Grube, B., Röske, Y., De Costa, P., & Chong, P.-W. (2024). Proprietary alpha-amylase inhibitor formulation from white kidney bean (Phaseolus vulgaris L.) promotes weight and fat loss: a 12-week, double-blind, placebo-controlled, randomized trial. In Scientific Reports (Vol. 14, Issue 1). Springer Science and Business Media LLC. https://doi.org/10.1038/s41598-024-63443-8
32. Houghton, D., Shannon, O. M., Chater, P. I., Wilcox, M. D., Pearson, J. P., Stanforth, K., Jordan, C., Avery, L., Blain, A. P., Joel, A., Jeffers, R., Nolan, R., Nelson, A., Stewart, C. J., & Malcomson, F. C. (2023). White kidney bean extract as a nutraceutical: effects on gut microbiota, alpha-amylase inhibition, and user experiences. In Gut Microbiome (Vol. 4). Cambridge University Press (CUP). https://doi.org/10.1017/gmb.2023.5
33. Aydar, E. F., Mertdinç, Z., Demircan, E., Koca Çetinkaya, S., & Özçelik, B. (2023). Kidney bean (Phaseolus vulgaris L.) milk substitute as a novel plant-based drink: Fatty acid profile, antioxidant activity, in-vitro phenolic bio-accessibility and sensory characteristics. In Innovative Food Science & Emerging Technologies (Vol. 83, p. 103254). Elsevier BV. https://doi.org/10.1016/j.ifset.2022.103254
34. Windahl, K., Chesnaye, N. C., Irving, G. F., Stenvinkel, P., Almquist, T., Lidén, M. K., Drechsler, C., Szymczak, M., Krajewska, M., de Rooij, E., Torino, C., Porto, G., Caskey, F. J., Wanner, C., Jager, K. J., Dekker, F. W., Evans, M., Windahl, K., … Evans, M. (2024). The safety of a low-protein diet in older adults with advanced chronic kidney disease. In Nephrology Dialysis Transplantation (Vol. 39, Issue 11, pp. 1867–1875). Oxford University Press (OUP). https://doi.org/10.1093/ndt/gfae077
35. Aydogdu, T., O’Mahony, J. A., & McCarthy, N. A. (2023). pH, the Fundamentals for Milk and Dairy Processing: A Review. In Dairy (Vol. 4, Issue 3, pp. 395–409). MDPI AG. https://doi.org/10.3390/dairy4030026
36. Qin, X., Cheng, J., Qi, X., Guan, N., Chen, Q., Pei, X., Jiang, Y., Yang, X., & Man, C. (2023). Effect of Thermostable Enzymes Produced by Psychrotrophic Bacteria in Raw Milk on the Quality of Ultra-High Temperature Sterilized Milk. In Foods (Vol. 12, Issue 20, p. 3752). MDPI AG. https://doi.org/10.3390/foods12203752
37. Neji, C., Semwal, J., Kamani, M. H., Máthé, E., & Sipos, P. (2022). Legume Protein Extracts: The Relevance of Physical Processing in the Context of Structural, Techno-Functional and Nutritional Aspects of Food Development. In Processes (Vol. 10, Issue 12, p. 2586). MDPI AG. https://doi.org/10.3390/pr10122586
38. Kong, C., Zhang, Y., Hu, Y., Duan, C., Yan, Z., & Zhou, S. (2025). Stabilization mechanism of white kidney bean based milk through novel perspectives of endogenous starch. In Food Chemistry: X (Vol. 25, p. 102192). Elsevier BV. https://doi.org/10.1016/j.fochx.2025.102192
39. Milovanovic, B., Djekic, I., Miocinovic, J., Djordjevic, V., Lorenzo, J. M., Barba, F. J., Mörlein, D., & Tomasevic, I. (2020). What Is the Color of Milk and Dairy Products and How Is It Measured? In Foods (Vol. 9, Issue 11, p. 1629). MDPI AG. https://doi.org/10.3390/foods9111629
40. Milovanovic, B. R., Djekic, I. V., Tomović, V. M., Vujadinović, D., & Tomasevic, I. B. (2022). Color measurement of animal source foods. In Theory and practice of meat processing (Vol. 6, Issue 4, pp. 311–319). The Gorbatov’s All-Russian Meat Research Institute. https://doi.org/10.21323/2414-438x-2021-6-4-311-319
41. Chero, M. J. S., Zamora, W. R. M., Chero, J. A. S., & Villarreyes, S. S. C. (2020). Application of the Computer Vision System to the Measurement of the CIE L*a*b* Color Parameters of Fruits. In Advances in Intelligent Systems and Computing (pp. 341–347). Springer International Publishing. https://doi.org/10.1007/978-3-030-51328-3_47
42. Ali, H. M., Ahmed, J. A. O., & El Zubeir, I. E. M. (2022). The effect of pH on the rheological and physical properties of analogue pizza cheese. In Journal of Food Science and Technology (Vol. 60, Issue 2, pp. 692–700). Springer Science and Business Media LLC. https://doi.org/10.1007/s13197-022-05654-3
43. Aydeniz Guneser, B. (2022). Characterization of physicochemical, rheological, aroma, and sensory properties of spreadable processed cheese supplemented with chia, quinoa, and teff seeds. In Mljekarstvo (Vol. 73, Issue 1, pp. 27–37). Croatian Dairy Union. https://doi.org/10.15567/mljekarstvo.2023.0104
44. Wang, J., Kuang, H., Zhang, Z., Yang, Y., Yan, L., Zhang, M., Song, S., & Guan, Y. (2020). Generation of seed lipoxygenase-free soybean using CRISPR-Cas9. In The Crop Journal (Vol. 8, Issue 3, pp. 432–439). Elsevier BV. https://doi.org/10.1016/j.cj.2019.08.008
45. Karolkowski, A., Belloir, C., Briand, L., & Salles, C. (2023). Non-Volatile Compounds Involved in Bitterness and Astringency of Pulses: A Review. In Molecules (Vol. 28, Issue 8, p. 3298). MDPI AG. https://doi.org/10.3390/molecules28083298
46. Silva, A. R. A., Silva, M. M. N., & Ribeiro, B. D. (2020). Health issues and technological aspects of plant-based alternative milk. In Food Research International (Vol. 131, p. 108972). Elsevier BV. https://doi.org/10.1016/j.foodres.2019.108972
47. Daszkiewicz, T., Michalak, M., & Śmiecińska, K. (2024). A comparison of the quality of plain yogurt and its analog made from coconut flesh extract. In Journal of Dairy Science (Vol. 107, Issue 6, pp. 3389–3399). American Dairy Science Association. https://doi.org/10.3168/jds.2023-24060
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